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Identification and Quantification of Hepatic Acid Metabolism in a Lipogenic Methionine/Choline-Deficient Diet-Fed Animal Model with Spin-Spin Relaxation Time by Using In Vivo Magnetic Resonance Spectroscopy
KH Song*, CH Yoo , SI Lim , MY Lee , BY Choe , Department of Biomedical Engineering, and Research Institute of Biomedical Engineering, College of Medicine, The Catholic University of Korea
Presentations
TU-C1-GePD-IT-1 (Tuesday, August 1, 2017) 9:30 AM - 10:00 AM Room: Imaging ePoster Theater
Purpose: The aims of this study were to quantify the hepatic lipid contents and composition of triglycerides in a preclinical nonalcoholic steatohepatitis (NASH) model during the progression of steatohepatitis with potential biomarkers, including spin-spin relaxation time, and in vivo proton magnetic resonance spectroscopy.
Methods: Weight-matched C57BL/6J mice were housed in standard plastic cages with ad libitum access to water. Steatohepatitis was induced with a methionine/choline-deficient (MCD) diet. The examinations were performed on a Bruker 9.4 T scanner. We used a stimulated echo acquisition mode sequence (repetition time/ mixing time/echo time = 3,500/10/20 ms). The T2 relaxations were estimated in MCD-fed mice with a fixed repetition time of 5,000 ms and multiple echo times of 20-70 ms.
Results: Our results indicated that the relaxation times (~2.03 ppm, p < 0.05; ~2.25 ppm, p < 0.001; ~2.78 ppm, p < 0.01) of triglycerides with lipid accumulation were altered in the livers of the MCD-fed mice. The results of the present study suggested that unsaturated fatty acids (total unsaturated fatty acids: p < 0.05; total unsaturated bond index: p < 0.01) were upregulated, while the polyunsaturated bond index was normal in the chronic NASH model.
Conclusion: These findings supported that fatty acid metabolism in hepatic steatosis in NASH induced by a MCD diet was distinguishable from progressive nonalcoholic fatty liver disease with in vivo spectra quantification and relaxation measurement, which can be used to effectively quantify lipid contents and characterize NASH.
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