Program Information
Determination of Bone Sodium (Na) and Na Exchange in Pig Leg Using In Vivo Neutron Activation Analysis (IVNAA)
M Coyne*, A Lobene , X Zhang , M Joo , C Neumann , P Lachcik , C Weaver , L Nie , Purdue University, West Lafayette, IN
Presentations
WE-RAM2-GePD-IT-4 (Wednesday, August 2, 2017) 10:00 AM - 10:30 AM Room: Imaging ePoster Theater
Purpose: The locations of sodium storage and its exchange mechanisms in different tissues in the body are not well known. This information is important for understanding the impact of sodium intake, absorption, and retention on human health, especially on the risk of developing chronic diseases like hypertension. The purpose of this study was to investigate the application of a compact deuterium-deuterium (DD) neutron generator-based IVNAA system in sodium nutrition studies.
Methods: The right posterior legs of two live pigs, one on a low sodium diet and one on a high sodium diet, both for 11 days, were irradiated inside a customized irradiation cave for 10 minutes (4.5 rem neutron dose to the leg) and then measured with a 100% high efficiency high purity germanium detector (HPGe). The spectra were analyzed to obtain the net Na counts at different time points. Bone Na concentrations were calculated using the calibration line created by irradiating sodium bone phantoms under the same conditions as the live pigs.
Results: The results show that the difference in concentration between the pigs on high vs low sodium diets was 335 +/- 127 ppm (p=0.01). Analysis also shows rapid exchange of sodium in the leg during the first 2 hour measurements, while the exchange was minimal at the second and third 2 hour measurements, taken 7 and 21 hours post irradiation. Applying saturation, decay and counting corrections to the measured sodium activities, the exchange decay time of sodium in the leg is 1.3 hours for the first two hours, and stable between 2-21 hours.
Conclusion: With these results, we conclude there is a non or low exchangeable compartment (likely to be bone) for sodium storage and that the DD neutron generator-based IVNAA is a useful method in sodium nutrition studies.
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