Program Information
Transverse Relaxation Time in Methylene Protons of Non-Alcoholic Fatty Liver Disease Rats
K-H Song1*, D-W Lee1 , B-Y Choe1 , (1) Department of Biomedical Engineering, Research Institute of Biomedical Engineering, College of Medicine, The Catholic University of Korea, Seoul, Seoul, Korea
Presentations
SU-E-I-64 (Sunday, July 12, 2015) 3:00 PM - 6:00 PM Room: Exhibit Hall
Purpose: The aim of this study was to evaluate transverse relaxation time of methylene resonance compared to other lipid resonances.
Methods: The examinations were performed using a 3.0 T scanner with a point - resolved spectroscopy (PRESS) sequence. Lipid relaxation time in a lipid phantom filled with canola oil was estimated considering repetition time (TR) as 6000 msec and echo time (TE) as 40 – 550 msec. For in vivo proton magnetic resonance spectroscopy (¹H - MRS), eight male Sprague – Dawley rats were given free access to a normal - chow (NC) and eight other male Sprague-Dawley rats were given free access to a high - fat (HF) diet. Both groups drank water ad libitum. T₂ measurements in the rats’ livers were conducted at a fixed TR of 6000 msec and TE of 40 – 220 msec. Exponential curve fitting quality was calculated through the coefficients of determination (R²).
Results: A chemical analysis of phantom and liver was not performed but a T₂ decay curve was acquired. The T₂ relaxation time of methylene resonance was estimated as follows: NC rats, 37.07 ± 4.32 msec; HF rats, 31.43 ± 1.81 msec (p < 0.05). The extrapolated M0 values were higher in HF rats than in NC rats (p < 0.005).
Conclusion: This study of ¹H-MRS led to sufficient spectral resolution and signal - to - noise ratio differences to characterize all observable resonances for yielding T₂ relaxation times of methylene resonance. ¹H - MRS relaxation times may be useful for quantitative characterization of various liver diseases, including fatty liver disease.
Funding Support, Disclosures, and Conflict of Interest: This study was supported by grant (2012-007883 and 2014R1A2A1A10050270) from the Mid-career Researcher Program through the NRF funded by Ministry of Science. In addition, this study was supported by the Industrial R&D of MOTIE/KEIT (10048997, Development of the core technology for integrated therapy devices based on real-time MRI-guided tumor tracking).
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